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. Author manuscript; available in PMC: 2021 Apr 2.
Published in final edited form as: Cell Stem Cell. 2020 Jan 30;26(4):593–608.e8. doi: 10.1016/j.stem.2019.12.009

Figure 1. Global transcriptomic time series reveals the kinetics of developing primary human alveolar epithelial type 2 cells.

Figure 1.

(A) The 5 stages of human lung development and samples obtained for bulk RNA sequencing.

(B) Principal component analysis (PCA) of gene expression across all 13 samples including primordial lung progenitors (PLP) derived from pluripotent stem cells at day 15 of differentiation, primary early human fetal lung alveolar epithelium (HFL; 16–17.5 weeks gestation), late HFL alveolar epithelium (20–21 weeks gestation), and adult alveolar epithelial type 2 cells (AEC2) sorted on the antibody HTII-280. The loadings of highly variable genes associated with differentiation and maturation of AEC2 in panel C, are overlayed on the PCA plot in B to indicate their weight on PC1 and PC2. Arrow tips denote the correlation coefficient of the respective gene with each principal component.

(C) Heatmap showing unsupervised hierarchical clustering of the top 1,000 most variable genes across all samples.

(D) Smoothed regressions of time series samples indicating normalized gene expression values (from panel C) for 8 selected genes associated with differentiation of AEC2, 6 genes associated with maturation of AEC2 and 8 selected downregulated AEC2 maturation and Wnt pathway genes.

See also Table S1