FIGURE 3.

AXL reverses tumor suppressor phenotypes mediated by Yes‐associated protein (YAP) silencing in vitro. A, B, EdU and CCK‐8 assay was performed after cotransfection with si‐YAP and the AXL plasmid for 24 h. Scale bars: 200 μm. C, Colony formation assay was performed after cotransfection with si‐YAP and AXL plasmid. D, E, Cell migration was assessed with wound healing and transwell assay after YAP silencing and AXL overexpression for 24 h. F, Cell invasion was performed using transwell assay with coated Matrigel cotransfection with si‐YAP and AXL plasmid. G, MMP2 and MMP9 were detected after cotransfection with si‐YAP and AXL plasmid for 48 h. *P < .05, **P < .01, ***P < .001, according to one‐way ANOVA