FIGURE 5.

Targeting relationship between long noncoding RNA 85 (lnc85) and microRNA (miR)‐324‐5p. A, miR‐324‐5p putative binding site on lnc85. B, Relative luciferase activity was detected in HepG2 and Huh7 cells cotransfected with pmirGLO‐lnc85‐WT or pmirGLO‐lnc85‐MUT vectors and mir‐324‐5p mimic or miR‐negative control (miR‐NC). C, D, Expression level of lnc85 and miR‐324‐5p before (C) and after (D) silencing of lnc85 in HL‐7702, HepG2, and Huh7 cells. Data are expressed as the mean ± SD of 3 independent experiments. *P ≤ .05, **P ≤ .005, ***P ≤ .0005 vs. control