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. 2020 Jul 5;111(9):3338–3349. doi: 10.1111/cas.14516

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© 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Scarcity of long noncoding RNA 85 (lnc85) enhances the suppression ability of microRNA (miR)‐324‐5p to its downstream gene. A‐C, mRNA expression of proliferation genes (A), antiapoptosis gene (B), and premigration genes (C) is decreased when lnc85 is silenced. D, E, Western blot images (D) and quantification (E) show downregulation of proliferation proteins after silencing of lnc85. F, Working model of lnc85 in hepatocellular carcinoma. Data are expressed as mean ± SD of 3 independent experiments. *P ≤ .05, **P ≤ .005 vs. siNC (si‐negative control)