Figure 3.

miR‐1908 directly regulates the expression levels of spermidine synthase (SRM). A, Summary of miR‐1908 target sites and mutated sites (shown in red) in the 3′‐UTR of SRM. B, Target validation of SRM was performed using a luciferase reporter assay. Values are depicted as fold change relative to the negative control miRNA (control). The values represent mean ± SE (n = 3). **P < .01; and n.s. indicates not significant. C, Effect of miR‐1908 on the expression levels of the target genes in 22Rv1 cells. The values are depicted as fold change relative to the negative control miRNA (control). The values represent mean ± SE (n = 3). **P < .01. D, Immunoblot analysis of 22Rv1 cells transfected with miR‐1908 mimic or negative control miRNA (control). 10 µg of the cell lysates derived from the transfected PCa cells was loaded for detection of SRM and actin