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. 2020 Jul 18;111(9):3258–3267. doi: 10.1111/cas.14535

Figure 4.

Figure 4

Sspermidine synthase (SRM) promotes the secretion of extracellular vesicles in prostate cancer cells. A, Representative images of LysoTracker staining in control and SRM knockdown 22Rv1 cells. Scale bars, 10 μm. The number of vesicles in 22Rv1 cells was counted. Values represent mean ± SE (n = 3). B, Confocal immunofluorescence micrographs of 22Rv1 cells transfected with SRM siRNA. Cells were stained with anti‐CD63 antibody. Scale bars, 10 µm. The number of vesicles in PCa cells was counted. The values represent mean ± SE (n = 5). C, Schematic model of the regulation of extracellular vesicle (EV) secretion in PCa. The novel signaling network of miR‐1908 and its target SRM regulating EV secretion in PCa cells. In the normal cells, expression of miR‐1908 was high, therefore expression of SRM was suppressed. However, because of downregulation of miR‐1908 in PCa cells, expression of SRM was upregulated and led to the acceleration of EV secretion from PCa cells, resulting in the promotion of cancer malignancy. Therefore, targeting SRM in PCa could be a novel therapeutic target for PCa