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. Author manuscript; available in PMC: 2020 Sep 3.
Published in final edited form as: Methods Mol Biol. 2012;833:153–176. doi: 10.1007/978-1-61779-477-3_11

Fig. 2.

Fig. 2.

Processing steps in qualitative FRAP. (a) Image of cells before and after the photobleach. Circles indicate the different measurement regions: solid gray – bleached region, solid black – correction region, dashed gray – background region. (b) Averaged fluorescence intensity from each of the regions in (a). Note that the bleached and correction regions have comparable brightness and that the background region does not show observational photobleaching. (c) The corrected FRAP curve is obtained by first correcting for the loss of fluorescence due to both intentional photobleaching and observational photobleaching, and then renormalizing the resulting curve by its pre-bleach intensity. (d) Application of qualitative FRAP to the glucocorticoid receptor (GR). Treatment of the cells with a proteasome inhibitor leads to a slowdown of the FRAP (8).