Figure 2.

A nucleosome array undergoes LLPS. A, DNA has a negatively charged phosphate backbone, while the core histone proteins are enriched with positively charged amino acids. The nucleosome is formed by electrostatic interactions between DNA and the histone octamer, which may halve the negative charge of DNA. B, A nucleosome array undergoes LLPS upon cation or histone H1 addition in vitro. C, Interphase chromatin forms a large and irregular assembly that behaves like a “liquid droplet” in the nucleus. Metaphase chromosomes are coated with Ki‐67 (orange), which serves as a surfactant