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. Author manuscript; available in PMC: 2020 Sep 3.
Published in final edited form as: Mol Cancer Res. 2012 Apr 11;10(6):787–799. doi: 10.1158/1541-7786.MCR-11-0462

Figure 6.

Figure 6.

Endogenous c-myc gene splicing was altered in colon cancer tissues. Total RNA was extracted from 5 matched, operatively excised human tumor samples (T) and adjacent nontumor epithelial tissue (N). cDNA was synthesized from RNA. c-myc intron 1 was examined to determine the extent of transcription by RT-PCR, through changing the reverse primers located in c-myc intron 1 (R2–R6). A, primers for detection of transcribed c-myc intron 1 are listed in Supplementary Table S2. c-myc intron 1 was disturbed and transcribed in (T) but not in (N). PC: positive control (cDNA synthesized by HeLa cell total mRNA); NC: negative control (H2O). B, treatment with SSA (500 ng/mL) disturbed transcription of c-myc intron 1. Transcription of c-myc intron 1 was also apparently disturbed by siRNA against SAP155 (C) and FIR (D). RNAs: PCR was carried out with primers F-R1 and F-R6 with 1 μg of extracted total RNA as a template to exclude genomic DNA contamination.