Figure 5:

Rare cell isolation of spiked CTC clusters. To demonstrate gentle isolation of rare cancer cell clusters, small numbers of clusters (five doublets and/or three triplets, in alternate colored fluorescent pre-labels) were manually spiked into each of nine runs on the integrated three-stage device of Fig. 1B (15.7 mL blood per run on average). After scanning the whole product, overall end-to-end yields are reported at left for MGH BRx-142 and LnCAP cells. Individual input-output counts are given at right, where empty circles represent missing cells in the product, and gray circles indicate single cells shed from spiked CTC clusters. 100% yield at top.