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. 2020 Aug 31;8:e9735. doi: 10.7717/peerj.9735

Figure 1. Influence of a range of annealing temperatures in the efficiency of amplification for two close related viruses by double PCR.

Figure 1

M: DL2000 DNA Marker; 1: 50 °C; 2: 52 °C; 3: 54 °C; 4: 56.4 °C; 5: 58.8 °C; 6: 61.2 °C; 7: 63.6 °C; 8: 66 °C; 9: 68 °C; 10: 70 °C; 11: negative control.