Table 1. Primers for PCV2 and PCV3 specific target gene amplification by the double PCR.
Virus | Primer sequence (5′ → 3′) |
Product length (bp) | Annealing temperature (°C) |
---|---|---|---|
PCV2 | F: ATAGGGGTCATAGGTTAGGGCATT | 305 | 56 |
R: GAAAAATGGCATCTTCAACACCCG | |||
PCV3 | F: GGAGGTTCACTAAGGTTGTTTGTT | 767 | 58 |
R: ACCACTTCATTACCCGCCTAAACGA |