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. 2020 Aug 31;8:e9735. doi: 10.7717/peerj.9735

Table 1. Primers for PCV2 and PCV3 specific target gene amplification by the double PCR.

Virus Primer sequence
(5′ → 3′)
Product length (bp) Annealing temperature (°C)
PCV2 F: ATAGGGGTCATAGGTTAGGGCATT 305 56
R: GAAAAATGGCATCTTCAACACCCG
PCV3 F: GGAGGTTCACTAAGGTTGTTTGTT 767 58
R: ACCACTTCATTACCCGCCTAAACGA