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. Author manuscript; available in PMC: 2020 Sep 3.
Published in final edited form as: Arch Pathol Lab Med. 2015 Jun 5;139(11):1334–1348. doi: 10.5858/arpa.2014-0498-RA

Figure 3. Invadopodial matrix degradation assay.

Figure 3.

UMSCC1 cells, a human oral cavity squamous cell carcinoma cell line, were plated onto an Alexa Fluor-405 labeled gelatin matrix. After 4 hours the cells were fixed and stained for cortactin and Tks5, two invadopodia markers. Representative images of the degraded fluorescent matrix (A) Tks5 (B) and cortactin (C) and the merged (D) (cortactin: red, Tks5: green, matrix: blue) staining at 60X magnification. The merged image shows colocalization of Tks5- and cortactin-rich structures associated with degradation holes in the Alexa Fluor-405 matrix.