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. 2020 Jul 14;133(15):1774–1785. doi: 10.1097/CM9.0000000000000917

Figure 3.

Figure 3

MiR-485-5p regulates MUC1 expression to facilitate the development of hepatocellular carcinoma. (A) Inhibition of miR-485-5p on HepG2 siMUC1 cell viability was detected by CCK-8. ∗P < 0.001 compared with siControl; †P < 0.01 compared with control group (siMUC1 + NC-miRNA). (B) Inhibition of miR-485-5p on the migration ability of HepG2 siMUC1 cells was analyzed by Transwell assays. ∗P < 0.001 compared with siControl; †P < 0.001 compared with control group (siMUC1 + NC-miRNA). (C) Inhibition of miR-485-5p on the invasion ability of HepG2 siMUC1 cells was analyzed by Transwell assays. ∗P < 0.001 compared with siControl; †P < 0.01 compared with control group (siMUC1 + NC-miRNA). (D) Promotion of miR-485-5p on HepG2 siMUC1 cell apoptosis was analyzed by flow cytometry. ∗P < 0.01 compared with siControl; †P < 0.05 compared with control group (siMUC1 + NC-miRNA). (E) Western blot showed the expressions of MMP-2, MMP-9, BAX, BCL2, and MUC1 in MUC1-silenced HepG2 cells with down-regulated miR-485-5p. The data in (A–D) are shown as mean ± standard deviation (n = 3). BAX: Bcl-2-associated X-protein; BCL2: B-cell lymphoma-2; CCK-8: Cell counting kit-8; MiR: MicroRNA; MMP: Matrix metalloproteinase; MUC1: Mucin 1.