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. 2020 Jul 14;133(15):1774–1785. doi: 10.1097/CM9.0000000000000917

Figure 5.

Figure 5

CircHECTD1 positively regulates MUC1 by sponging miR-485-5p. (A) MUC1 expressions measured by western blot and qRT-PCR after transfection with circHECTD1. ∗P < 0.001 compared with vector control. (B) MUC1 levels in circHECTD1-silenced HepG2 cells, detected by western blot and qRT-PCR. ∗P < 0.01 compared with siControl group. (C) Inhibition of miR-485-5p on HepG2 siHECTD1 cell viability was detected by CCK-8. ∗P < 0.01 compared with siControl, †P < 0.01 compared with control group (siHECTD1 + NC-miRNA). (D) Inhibition of miR-485-5p on HepG2 siHECTD1 cell migration capacity was analyzed by Transwell assays. ∗P < 0.001 compared with siControl; †P < 0.01 compared with control group (siHECTD1 + NC-miRNA). (E) Inhibition of miR-485-5p on HepG2 siHECTD1 cell invasion capacity was analyzed by Transwell assays. ∗P < 0.001 compared with siControl, †P < 0.01 compared with control group (siHECTD1 + NC-miRNA). (F) Promotion of miR-485-5p on HepG2 siHECTD1 cell apoptosis was tested by flow cytometry. ∗P < 0.01 compared with siControl; †P < 0.05 compared with control group (siHECTD1 + NC-miRNA). (G) Western blot showed the expressions of MMP2, MMP9, BAX, BCL2, and MUC1 in circHECTD1-silenced HepG2 cells with downregulated miR-485-5p. The data in (A–F) are shown as mean ± standard deviation (n = 3). BAX: Bcl-2-associated X-protein; BCL2: B-cell lymphoma-2; CCK-8: Cell counting kit-8; MiR: MicroRNA; MMP: Matrix metalloproteinase; MUC1: Mucin 1; qRT-PCR: Quantitative real-time polymerase chain reaction.