Table 1. Macromolecule production.

Source organism	D. rerio
DNA source	HDAC6 gene from D. rerio (UniProt F8W4B7; CD1 residues 61–419)
Cloning vector	pET-28a
Expression vector	pET-28a
Expression host	E. coli
Complete amino-acid sequence of the construct produced†
K330L HDAC6 CD1	MGSSHHHHHHSSGLVPRGSHM TGTGLVYVDAFTRFHCLWDASHPECPARVSTVMEMLETEGLLGRCVQVEARAVTEDELLLVHTKEYVELMKSTQNMTEEELKTLAEKYDSVYLHPGFFSSACLSVGSVLQLVDKVMTSQLRNGFSINRPPGHHAQADKMNGFCMFNNLAIAARYAQKRHRVQRVLIVDWDVHHGQGIQYIFEEDPSVLYFSVHRYEDGSFWPHLKESDSSSVGSGAGQGYNINLPWNKVGMESGDYITAFQQLLLPVAYEFQPQLVLVAAGFDAVIGDPLGGMQVSPECFSILTHMLKGVAQGRLVLALEGGYNLQSTAEGVCASMRSLLGDPCPHLPSSGAPCESALKSISKTISDLYPFWKSLQTFE
H82F/F202Y HDAC6 CD1	MGSSHHHHHHSSGLVPRGSHM TGTGLVYVDAFTRFHCLWDASFPECPARVSTVMEMLETEGLLGRCVQVEARAVTEDELLLVHTKEYVELMKSTQNMTEEELKTLAEKYDSVYLHPGFFSSACLSVGSVLQLVDKVMTSQLRNGFSINRPPGHHAQADKMNGYCMFNNLAIAARYAQKRHRVQRVLIVDWDVHHGQGIQYIFEEDPSVLYFSVHRYEDGSFWPHLKESDSSSVGSGAGQGYNINLPWNKVGMESGDYITAFQQLLLPVAYEFQPQLVLVAAGFDAVIGDPKGGMQVSPECFSILTHMLKGVAQGRLVLALEGGYNLQSTAEGVCASMRSLLGDPCPHLPSSGAPCESALKSISKTISDLYPFWKSLQTFE

^†The His-tag sequence is underlined. The CD1 sequence starts with Thr61 (bold). Mutated amino acids are shown in bold and underlined.