| Method |
Sitting-drop vapor diffusion |
| Plate type |
PS MRC crystallization plate |
| Temperature (K) |
100 |
| Protein concentration (mg ml−1) |
10 |
| Buffer composition of protein solution |
50 mM HEPES pH 7.5, 100 mM KCl, 1 mM TCEP, 5% glycerol, 2 mM inhibitor |
| Composition of reservoir solution |
| Trichostatin A complex |
0.2 M potassium acetate, 20% PEG 3350 |
| SAHA-BPyne complex |
0.2 M potassium sodium tartrate tetrahydrate, 20% PEG 3350 |
| 4-Iodo-SAHA complex |
0.2 M magnesium chloride hexahydrate, 20% PEG 3350 |
| Volume and ratio of drop |
1:1 ratio of protein:precipitant solution |
| Volume of reservoir (µl) |
80 |
