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. 2020 Aug 19;76(Pt 9):406–413. doi: 10.1107/S2053230X20010067

Table 2. Crystallization.

Method Vapor diffusion, sitting drop
Plate type 96-well plates
Temperature (K) 293
Protein concentration (mg ml−1) 30
Buffer composition of protein solution 4 mM NaCl, 5 mM Tris–HCl pH 8.0
Composition of reservoir solution 100 mM sodium acetate pH 4.6, 30%(w/v) PEG 400, 200 mM calcium acetate
Volume and ratio of drop 2.4 µl, 1:1
Volume of reservoir (µl) 100