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Fluorescence quenching spectra of SARS-CoV-2 3CLpro. A solution containing 2 μM SARS-CoV 3CLpro showed a strong fluorescence emission with a peak at 340 nm at the excitation wavelength of 295 nm. After adding 80 μM each inhibitory compound such as baicalin, herbacetin, and pectolinarin, fluorescence quenching spectra were obtained.
