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. 2020 Jul 13;35(1):1503–1512. doi: 10.1080/14756366.2020.1791841

Table 1.

Investigation of analogues of galactocerebroside as substrates of CSTa.

Compound Structure Percent conversiona at 100 µM Kma (µM) Vmaxa (µmol/min/mg protein) kcat/Kma
β-Glycosides          
 3 Galactosylceramideb graphic file with name IENZ_A_1791841_ILG0001_B.gif 100%c 60.318 0.073818 12318
 8 Psychosine graphic file with name IENZ_A_1791841_ILG0002_B.gif n.d. 10318 0.10518 10218
 9 Glucosylceramideb graphic file with name IENZ_A_1791841_ILG0003_B.gif 19% n.d. n.d. n.d.
 10 β-KRN7000 graphic file with name IENZ_A_1791841_ILG0004_B.gif 42% 550 0.113 20.5
α-Glycosides          
 11 graphic file with name IENZ_A_1791841_ILG0005_B.gif 40% 907 0.1080 11.9
 12 graphic file with name IENZ_A_1791841_ILG0006_B.gif 30% 358 0.0449 12.5
 13 graphic file with name IENZ_A_1791841_ILG0007_B.gif 22% 392 0.0584 15.0
 14 graphic file with name IENZ_A_1791841_ILG0008_B.gif 32% 493 0.0590 12.0
 15 graphic file with name IENZ_A_1791841_ILG0009_B.gif 20% n.d. n.d. n.d.
 16 graphic file with name IENZ_A_1791841_ILG0010_B.gif 9% n.d. n.d. n.d.
 17 graphic file with name IENZ_A_1791841_ILG0011_B.gif 25% 521 0.0519 10.0
 18 KRN7000 graphic file with name IENZ_A_1791841_ILG0012_B.gif 51% 438 0.0758 17.3
 19 graphic file with name IENZ_A_1791841_ILG0013_B.gif 32% 751 0.0834 11.1
 20 graphic file with name IENZ_A_1791841_ILG0014_B.gif 15% n.d. n.d. n.d.
 21 graphic file with name IENZ_A_1791841_ILG0015_B.gif 16% n.d. n.d. n.d.
 22 graphic file with name IENZ_A_1791841_ILG0016_B.gif 19% n.d. n.d. n.d.

aStandard errors were typically below 30% of the reported mean values.

bR: saturated or unsaturated alkyl or hydroxyalkyl residue (C16–C27).

cConversion of physiological substrate was set at 100%.

Km and Vmax values are shown in bold.