Figure 2.

Neuroprotective effects of HSYA in PD mice model. Mice were injected with 6-OHDA into the right striatum in the absence or presence of HSYA at 2, 4, and 8 mg/kg, respectively. (A) APO-induced rotation was measured as the average circle number per 10 min for a period of 30 min following subcutaneous APO injection (n = 15 in each group). (B and C) Western blotting was performed to evaluate the expression of TH protein in the SN (B) and STR (C) (n = 5 in each group). (D and E) Brain tissue sections were immunohistochemically stained for TH in both the SN (D) and STR (E). The graph bars show the number of TH-positive neurons in the SN (D) and the optical density of TH positive terminals in the STR (E). The data are presented as the means ± S.E.M (n = 5 in each group). *p < 0.05 vs. sham group; ^#p < 0.05 vs. 6-OHDA group.