Figure 4.

Curcumin suppressed EMT and inflammation via the TLR4/NF-κB pathway in UUO mice. (A) Expression of E-cadherin, vimentin and TLR4 in mouse renal sections, detected by immunochemical staining. (200× magnification, bar = 50 μm). The IHC evaluation was performed by two independent observers, who were blind to the slice. The staining intensity was scored as (1) 0 point: negative; (2) 1 point: weak positive; (3) 2 points: moderated positive; (4) 3 points: strong positive. (B) The expression of E-cadherin and vimentin in UUO mice, as evidenced by qPCR (n = 4). (C–D) qPCR and western blotting analyses of the expression of TGF-β1 and TLR4 in UUO mice (n = 4). (E) The expression of NF-κB P65 in the renal tissue, detected by western blotting (n = 4). Cur, curcumin. *p < 0.05, **p < 0.01.