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. 2020 Aug 31;9(1):1809947. doi: 10.1080/2162402X.2020.1809947

Figure 3.

Figure 3.

β-Catenin inhibition upregulates the expression of CXCR3 chemokines in colorectal tumor cells. (a) qPCR analysis of chemokine mRNA expression in CT26 tumors of iCRT4- or vehicle-treated mice. n = 4. (b) qPCR analysis of chemokine mRNA expression in CT26 cells treated with iCRT14 or vehicle in vitro. n = 4. (c) Quantification of CXCL10 proteins in the media of CT26 cell culture by ELISA. n = 3. (d-e) CT26 (d) or MC38 (e) were transduced with lentivirus containing S33Y.β-catenin or empty vector. Expression of β-catenin protein and chemokine mRNA expression was measured by Western blot and qPCR respectively. n = 4. (f) MC38-S33Y.β-cat cells was treated with iCRT14 or vehicle. Chemokine mRNA expression was analyzed by qPCR. n = 4. (g) qPCR analysis of chemokine mRNA expression in HCT16 and SW480 cell lines treated with iCRT14 or vehicle in vitro. n = 4. * P < .05, ** P < .01, *** P < .001, **** P < .0001