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. 2020 Aug 30;9(1):1808424. doi: 10.1080/2162402X.2020.1808424

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The co-culture conditioned medium (CM) from slanMo and NK cells reduces the proliferation of melanoma cells. (A) Ki67 staining of SK-Mel-28 cultured with CM as described before. Quantification of Ki67⁺ cells. Representative image and cumulative data out of 11 donors. (B) Ki67 staining of SK-Mel-28 treated with recombinant TNF-α and IFN-γ and quantification of positive cells. Representative image out of 3 experiments. (C) ³H-Thymidine incorporation assay with SK-Mel-28 cells treated with CM as described before. 1 d after reseeding, the cells were pulsed with ³H-Thymidine for 12 h. Cumulative data out of 14 donors. (D) ³H-Thymidine incorporation assay with SK-Mel-28 cells treated with recombinant TNF-α and IFN-γ. Cumulative data from 4 experiments.