Figure 3.

Decreased lung tumor burden in MFP tumor-bearing mice is CD8⁺ T cell dependent. (a) Experimental overview. BALB/c mice were injected with depletion antibodies against CD8⁺ (CD8⁺ T cell depletion) or asialo-GM1 (NK cell depletion) before injection with 67NR tumor cells in the MFP and IV (MFP + Lung) or IV only (Lung), with repeated dosing of depletion antibodies as indicated. The same tumor injections were performed in NSG mice, without depletion antibodies. Mice were culled 10 days after tumor injection for lung metastasis enumeration and depletion check. (b) Representative plot of depletion check where spleens of BALB/c mice in (a) were harvested, after 4 doses of depletion antibodies, and stained with antibodies indicated for flow cytometry. (c,d) Representative images (c) and enumerated metastases in lungs of BALB/c and NSG mice as described in (a) Data points represent whole lungs from individual mice from 2 independent experiments with mean ± SEM (n = 4–6 mice per group, per experiment). Unpaired t-test. ns P ≥ 0.05; *P < .05.