FIG. 4.

MALDI-TOF mass spectra of O-linked glycosylation from rat spinal cord samples. Permethylated O-linked glycosylation from (A) control, (B) sham, and (C) 14 DPI samples derived from the 35% acetonitrile fraction (see Methods section). All molecular ions are [M+Na]⁺. Indicated areas (m/z 1300–2300) in the spectra have a 10-fold magnification. Putative structures are based on composition, tandem MS, and biosynthetic knowledge. Full methods and spectra for the 3 DPI samples can be found in the supplementary figures (Supplementary Fig. S4). DPI, days post-injury; MALDI-TOF, matrix-assisted laser desorption ionization time-of-flight; MS, mass spectrometry. Color image is available online.