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. 2020 Feb 10;2(3):acmi000096. doi: 10.1099/acmi.0.000096

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© 2020 The Authors

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Fig. 1. — PCR amplification of the 16S−23S rDNA ITS of K. pneumoniae . Strain K1 was used as a positive control for all runs. K. pneumoniae isolates (K2, K3, K7 and K9) produced a band at 260 bp with the Pf/Pr2 primer pair, in addition to a 130 bp band with the Pf/Pr1 primer pair. Non- K. pneumoniae isolates (K4, K5 and K6) only produced a 260 bp band with primer pair Pf/Pr2 and failed to produce a band with Pf/Pr1, whereas K8 did not produce a band with Pf/Pr2, but did with Pf/Pr1. L, 100 bp ladder.