Fig 7. A quantification of qICC γH2AX data of irreparable DNA damage in GB cells.

Cells were seeded at high densities (50,000 cells/cm²) and either left untreated or treated for five consecutive days with either 10 μM TMZ (T) or NEO212 (N) or 2 Gy (R) alone or combinations without (T+R or N+R) or with (T+R+O or N+R+O) Olaparib (O). Persistent γH2AX foci were digitally counted and expressed relative to the total number of cell nuclei. All ratio values (pixel counts from treatments expressed as a % ratio of persistent γH2AX foci to nuclei values normalized to untreated counts) were found highly statistically significant with p values of <0.001 (ANOVA with Tukey post-hoc testing).