Fig 9. FACS analysis of cell death resulting from irreparable DNA damage.

U251 and U251M cells were seeded at high densities (50,000 cells/cm²) and were either left untreated (UT) or treated for five consecutive days with either 10 μM TMZ (T) or NEO212 (N) or 2 Gy (R) alone or combinations without (T+R or N+R) or with (T+R+O or N+R+O) Olaparib (O). The cells were probed with a Pacific Blue-labeled Annexin V and then fixed, permeabilized and probed with an AF488-labeled γH2AX antibody. γH2AX/Annexin V double positive cells (i.e., dead cells due to irreparable DNA damage) are shown as percentages of total cell numbers. Representative panels are shown from three independent experiments.