Table 1.

Summary of minimum inhibitory concentrations (MICs), susceptibilities to various antibiotics (S, susceptible; I, intermediate; R, resistant)^a and associated antibiotic resistance genes identified through WGS analysis of five Ontario E. anophelis clinical isolates

a, The interpretive criteria applied were those of CLSI for non- Enterobacteriaceae (CLSI M100-S25) [25].

b, Accession numbers of PHOL-104.

c, Protein homologues of resistance genes were identified using the hmmscan function of HMMER3 (http://hmmer.org/) against the Core ResFams database [18], the specialty genes for antibiotic resistance identified by PATRIC [19] following RAST annotation [20], and/or the Resistance Gene Identifier (Perfect and Strict hits only) of CARD [21, 22].

d, Protein variant model antibiotic resistance gene targets were identified following gene annotation by Prokka [16].

e, All isolates had a serine at position 83 conferring susceptibility to fluoroquinolones [6, 28].