Table 1. Permeability of an in vitro BBB model to immunoglobulins and sucrose.

Permeability, median (IQR)	Control-IgG	NMO-IgG1	NMOIgG2	NMO-IgG3	p-value(Kruskal Wallis)
IgG (ul.hr -1. cm-2)	0.54 (0.35–0.73)	0.98 (0.54–1.45)	1.22 (1.12–1.75)*	1.17 (0.30–1.18)	0.238
Sucrose (ul.min -1. cm-2)	0.31 (0.29–0.34)	0.42 (0.36–0.51)*	0.44 (0.41–0.48)*	0.39 (0.33–0.39)	0.042

*p = 0.050 vs. Control IgG

Abbreviation; IQR, interquartile range

BMEC were exposed to three different NMO-IgG (2 mg/ml) in the upper compartment of the culture system simulating blood. The basolateral side simulates the CSF. NMO-IgG₂ clearance rate was twice that of the Control-IgG, p = 0.050. When exposing cells to the NMO-IgG or control-IgG, the sucrose clearance rate in cells treated with NMO-IgG₁ and NMO-IgG₂ was significantly higher than in those treated with Control-IgG, p = 0.050)