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. 2020 Sep 3;15(9):e0236336. doi: 10.1371/journal.pone.0236336

Table 2. Assessment of MAP infection status in 155 Holstein Friesian cows included in the study.

Diagnostic method Focal (n = 55) Multifocal (n = 18) Diffuse (n = 15) NL (n = 6) PTB-free farm (n = 61)
ZN 49.09% 94.44% 100.00% 0% UN
IDEXX ELISA 5.45% 27.78% 73.33% 0% 0%
FECAL PCR 9.09% 33.33% 73.33% 0% 0%
FECAL CULTURE 5.45% 16.67% 26.66% 0% 0%
TISSUE PCR 28.30% 44.44% 73.33% 0% UN
TISSUE CULTURE 28.30% 44.44% 66.66% 0% UN
CLINICAL SIGNS 0.00% 21.43% 64.28% 0% 0.00%

NL, refers to the 6 control animals with no PTB-associated lesions detected in their gut tissue; PTB-free farm; refers to the 61 control animals from a PTB-free farm; UN, undetermined (tissues not available as animals were alive); The calculation of the % of animals with clinical signs is based on animals with known clinical status (focal n = 28, multifocal n = 14, diffuse n = 14, NL n = 4 and PTB-free farm n = 61).