Fig. 1.

Conserved uORFs of strawberry bZIP genes. a Diagram depicting the sucrose-dependent post-transcriptional control of bZIP genes and the strategy for increasing sugar content by engineering the conserved sucrose control uORF (SC-uORF). The mutant sc-uorf reduced inhibition of translation of the bZIP gene, leading to increased sugar accumulation. uORF, upstream open reading frame. pORF, primary open reading frame. b Phylogenetic tree of the S1 group bZIP genes in Arabidopsis and strawberry. The strawberry S1 group bZIP homologs are shown in red. c Schematic illustration of the organization of the uORFs in the mRNA of the four strawberry bZIP genes. The uORFs are shown by the yellow lines with arrowheads upstream of the blue line with arrowhead representing the pORF. The longest uORF of each gene is the SC-uORF. d Alignment of the conserved SC-uORF amino acid sequences in Arabidopsis, strawberry, and other dicotyledonous and monocotyledonous plants. Black box with white letter, 100% identity; dark gray box with white letter, 80% identity; gray box with black letter, 60% identity