Table 3.

Characteristics of 12 compound microsatellite loci developed for A. venetum across all the samples. Shown for each locus are the locus name, the forward (F) and reverse (R) primer sequence, allele size, repeat motif, genetic characteristics and GenBank accession number

Locus	Forward	Reverse	Size (bp)	SSR motif	NA	HO	HE	PIC	HWE	GenBank accession number
Av02	AAAAATGGGCAATGGTGGGC	AGGCGTAGGTGAAGAGGAGT	224	(AT)11	5	0.515	0.511	0.462	NS	MT737291
Av08	AATCAGCCACCGAGTTACCG	ACCTCCTGCAAGCTGAATCC	220	(CAT)6	5	0.653	0.639	0.574	NS	MT737292
Av13	ACGAGAAGTTGGAAACAGACCA	GTATTTGGTGTCTTCGGCGC	269	(GT)6	3	0.050	0.049	0.048	ND	MT737293
Av15	ACTCGTTGGACATGATGTGCT	GGACCTTCTCATCAGCCTCG	208	(CT)8	3	0.228	0.204	0.184	ND	MT737294
Av21	AGCAGGGGAGAAGAATGCAC	GGGTCTTGATGAGGTGAGGG	250	(ACAA)6	4	0.109	0.114	0.110	ND	MT737295
Av33	CCAAACCACACAGCTCAACG	CCAAACCACACAGCTCAACG	180	(AT)9	3	0.337	0.339	0.312	ND	MT737296
Av39	CGCTTGCTGCCTCATCATTC	CCCTCTCACACCATCCCAAC	271	(TGC)6	3	0.545	0.651	0.575	NS	MT737297
Av43	CTGCATTCCCGCAAGTAACAG	TGATGCAGCTTAGGAGGGTC	258	(AAG)5	2	0.356	0.294	0.250	ND	MT737298
Av55	GCTCCGAGAAATCCTGCTCA	GCACTGCACCCTCCTACTAC	218	(AG)9	3	0.030	0.030	0.029	ND	MT737299
Av63	GGGTTTTGCTTCTGGGCATG	GAGCCAATCCGAACCCCAC	157	(TC)9	2	0.327	0.301	0.254	ND	MT737300
Av75	TCACTAGTACCCACCACCCC	AGTGGTGGCGTTGCTATGAA	212	(GT)6(AT)6	2	0.485	0.490	0.369	NS	MT737301
Av88	TGCATCATGTAGGGTACACACC	GCAAGTGTTCGCTGAGTTCC	159	(AAT)7	4	0.396	0.487	0.400	NS	MT737302
Mean					3.25	0.336	0.342	0.297

Notes: N_A number of alleles, H_O observed heterozygosity, H_E expected heterozygosity, PIC polymorphism information content, HWE Hardy-Weinberg equilibrium, NS Not significant for departure from HWE, ND Not detected for departure from HWE