. 2020 Jun 22;103(3):1009–1016. doi: 10.4269/ajtmh.20-0145

Table 3.

Primers and probes used for amplification and sequencing of SFG Rickettsia from Dermacentor variabilis and Amblyomma americanum

Organism	Primer/probe	Target	Sequence (5′-3′)	Reference
SFG Rickettsia	Pfmt-F2	n-f-met-23s-rRNA	AAGAGAGTAAAAAAGCTTTG	This study
SFG Rickettsia	Pspacer-R1	n-f-met-23s-rRNA	TGGGTTTGCCTCATATAGC	This study
SFG Rickettsia	Pglta-1F	gltA	ATTGCTTTACTTACGACCC	This study
SFG Rickettsia	Pglta-2R	gltA	TTCAAGTTCTATTGCTATTTG	This study
SFG Rickettsia	R190-70p	ompA	ATGGCGAATATTTCTCCAAAA	Ref. 31 and ⁵⁶
SFG Rickettsia	Rr190.602n	ompA	AGTGCAGCATTCGCTCCCCCT	Ref. 31 and ⁵⁶
SFG Rickettsia	R17K128F2	17kD	GGGCGGTATGAAYAAACAAG	30
	R17K238 R		CCTACACCTACTCCVACAAG
	R17K202 Probe		FAM-CCGAATTGAGAACCAAGTAATGC-MGB
Rickettsia amblyommatis	Ra477 F	ompB	GGTGCTGCGGCTTCTACATTAG	Ref. 57 and ²²
	Ra618 R-mod		CCATTAGTAACATTTAATGTACCGTTAACAC
	Ra532 Probe-mod		VIC-CACTTGGACAGAATGCTT-MGB
Rickettsia rickettsii	RR1370F	ompB	ATAACCCAAGACTCAAACTTTGGTA	Ref. 58^,⁵⁰, and his study
	RR1494R		GCAGTGTTACCGGGATTGCT
	RR1425B Probe		FAM-TTAAAGTTCCTAATGCTATAACCCTTACC-MGB

For the qPCR assays, we used MGB quencher instead of the TAMRA or BHQ1.