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. 2020 May 11;103(3):1076–1080. doi: 10.4269/ajtmh.20-0131

Figure 3.

Figure 3.

Molecular identification of the MHOM/BR/LCD/AAB-MA isolate. (A) ITS-1 gene amplification in 1% agarose gel. (B) Digestion of the amplified product with HaeIII and size separation in 4% agarose gel. (C) Phylogenetic tree of ITS-1 gene sequences from Leishmania species. Scale bar indicates 0.1% divergence. M = molecular weight marker (100 bp); (+) = positive control; (−) = negative control; 1 = clinical isolate; La =Leishmania amazonensis (IFLA/BR/1967/PH8); Lb = Leishmania braziliensis (MHOM/BR/1975/M2903).