Fig. 5.

Effects of decitabine (DEC)-treated dendritic cells (DCs) on T cell proliferation and associated molecules. a Splenic DCs isolated from recipient mice treated with or without DEC were co-cultured with donor T cells, and proliferation of CD3 + , CD3 + CD4 + , and CD3 + CD8 + T cells was assessed via the carboxyfluorescein diacetate succinimidyl ester method. b Changes in the expression of molecules following DEC administration assessed using a gene kit of 84 genes known to be involved in DC function, and c the dynamics of IFN-γ and CD28 expression over time analyzed by real-time polymerase chain reaction