Table 1.
Primers and probes for Plasmodium species detection
| Method | Species | Primer | Sequence (5′–3′) | Length (bp) | References |
|---|---|---|---|---|---|
| Nested PCR | Plasmodium sp. | rPLU1 | TCAAAGATTAAGCCATGCAAGTGA | 1670 | [43] |
| rPLU5 | CCTGTTGTTGCCTTAAACTTC | ||||
| P. falciparum | rFAL1 | TTAAACTGGTTTGGGAAAACCAAATATATT | 205 | ||
| rFAL2 | ACACAATGAACTCAATCATGACTACCCGTC | ||||
| P. vivax | rPVIV1 | CGCTTCTAGCTTAATCCACATAACTGATAC | 121 | ||
| rPVIV2 | ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA | ||||
| P. malariae | rMAL1 | ATAACATAGTTGTACGTTAAGAATAACCGC | 145 | ||
| rMAL2 | AAAATTCCCATGCATAAAAAATTATACAAA | ||||
| P. ovale | rOVA1WC | TGTAGTATTCAAACGCAGT | 659–662 | ||
| rOVA2WC | TATGTACTTGTTAAGCCTTT | ||||
| P. ovale curtisi | rOVA1 | ATCTCTTTTGCTATTTTTTAGTATTGGAGA | 800 | [44] | |
| rOVA2 | GGAAAAGGACACATTAATTGTATCCTAGTG | ||||
| P. ovale wallikeri | rOVA1v | ATCTCCTTTACTTTTTGTACTGGAGA | 780 | ||
| rOVA2v | GGAAAAGGACACTATAATGTATCCTAATA | ||||
| Real-time PCR | Plasmodium sp. | Plasmo 1 | GTTAAGGGAGTGAAGACGATCAGA | [35] | |
| Plasmo 2 | AACCCAAAGACTTTGATTTCTCATAA | ||||
| P. falciparum | P. fal-probe | FAM-AGCAATCTAAAAGTCACCTCGAAAGATGACT-TAMRA | |||
| P. vivax | P. v-probe | HEX-AGCAATCTAAGAATAAACTCCGAAGAGAAAATTCT-TAMRA | |||
| P. malariae | P. m-probe | FAM-CTATCTAAAAGAAACACTCAT-MGB | |||
| P. ovale | P. o-probe | HEX-CGAAAGGAATTTTCTTATT-MGB | |||
| P. ovale | POF | ATAAACTATGCCGACTAGGTT | [42] | ||
| POR | ACTTTGATTTCTCATAAGGTACT | ||||
| P. ovale curtisi | POC-probe | FAM-TTCCTTTCGGGGAAATTTCTTAGA-BHQ1 | |||
| P. ovale wallikeri | POW-probe | HEX-AATTCCTTTTGGAAATTTCTTAGATTG-BHQ1 |