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. 2020 Sep 3;148:46–49. doi: 10.1016/j.yjmcc.2020.08.017

Fig. 2.

Fig. 2

MiR-200c regulates Ace2 expression in NRCM and hiPSC-CM (A) Relative expression of Ace2 in NRCMs transfected with pre-miR-200b, −200c and −429 (n = 3, technical replicates); (B) Relative protein levels of Ace2 in NRCMs transfected with pre-miR-200b and -200c (n = 4, technical replicates); (C) Relative expression of miR-200c in hiPSC-CM transfected with pre-miR-200c (n = 3, technical replicates); (D) Relative expression of ACE2 in hiPSC-CM transfected with pre-miR-200c (n = 3, technical replicates); (D) Luciferase activity in HEK293T cells transfected with pre-miR-200c (or negative control) and ACE2 3’UTR luciferase reporter plasmids (WT or MUT) (N = 3, independent experiments).