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. 2020 Jan 13;41(5):661–669. doi: 10.1038/s41401-019-0325-6

Fig. 5. The transcription factor Sp1 regulates the promoter activity of ITGAV.

Fig. 5

a The representative binding site of KLF5 (red, TCTCCTCCCA) and Sp1 (green, TCCCCGCCCCG) on the −309/−16 bp region of the ITGAV promoter. b LX2 cells were transiently overexpressed with pcDNA3.1-SP1 (left) or pcDNA3.1-KLF5 (right) for 48 h, and real-time PCR for ITGAV and SP1 or KLF5. The data are expressed as the mean ± SD, *P < 0.05 compared with the pcDNA3.1 group. LX2 cells were transiently transfected with pcDNA3.1-SP1 (c) or siRNA-SP1 (d) for 48 h, followed by IMB-S7 for an additional 24 h. Western blot analysis for Sp1 and integrin αv. e Biotin-labeled 30-mer GC-rich oligonucleotides (biotin-Sp1) were incubated with LX2 cell lysis in the presence of IMB-S7 or not, and Western blot analysis was performed for Sp1 that specifically bound to the biotin probes.