Fig. 3.

Endophilin A2 regulates TMEM16A protein expression. a Endophilin A2 had no effects on TMEM16A mRNA level under overexpression (left) or knockdown (right) of Endophilin A2 in BASMCs. b Western blotting showed overexpression (up) of Endophilin A2 decreased TMEM16A expression, whereas knockdown (down) of Endophilin A2 increased TMEM16A expression in BASMCs. Multiplicity of infection (MOI): the number of infected viral particles per cell. n = 6, *P < 0.05 vs. Con. c Representative traces of calcium-activated Cl⁻ current, I–V curves, and mean current densities measured at ±100 mV in BASMCs isolated from wild-type (WT) and Endophilin A2 transgenic mouse (endo2^Tg). n = 8, *P < 0.05 vs. WT. d Expression of TMEM16A in basilar artery from WT and Endophilin A2 transgenic mouse (endo2^Tg). n = 6, *P < 0.05 vs. WT