Fig. 4.

Involvement of TMEM16A ubiquitination in TMEM16A protein quality control. a BASMCs were treated with Angiotensin II (500 nM) for 48 h, then ubiquitination of TMEM16A was detected by Western blotting. b TMEM16A ubiquitination in basilar artery isolated from sham operation (Sham) and hypertensive (Hyper) rats. c Overexpression Endophilin A2 increased TMEM16A ubiquitination in BASMCs. Cells were transfected with HA-TMEM16A and His-ubiquitin plasmids. Then, the cells were infected with Lacz or Endophilin A2 adenovirus as indicated, ubiquitination of TMEM16A was detected by Western blotting