Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | C57BL/6JRj | Janvier Labs | https://www.janvier-labs.com/en/fiche_produit/c57bl-6jrj_mouse/ | |
| Genetic reagent (M. musculus) | TRPV1-GCaMP3 | This paper | Obtained by crossing Gt(ROSA)26Sortm38(CAG-GCaMP3)Hze/J mice (Stock#: 029043) with Trpv1tm1(cre)Bbmmice (Stock#: 017769). Both strains were acquired from Jackson Laboratory. Crossings were made in house. | |
| Peptide, recombinant protein | WGA-AF647 (wheat germ agglutinin- Alexa Fluor 647) | Thermo Fisher Scientific | Cat#: W32466 | 0.8% in PBS; 10 µl per injection |
| Commercial assay or kit | RNAscope 2.0 Fluorescent Multiplex Reagent Kit | Advanced Cell Diagnostics | Cat#: 320850; RRID:SCR_012481 | |
| Commercial assay or kit | TRPV1 Probe | Advanced Cell Diagnostics | Cat#: 313331 | |
| Commercial assay or kit | TRPM3 Probe | Advanced Cell Diagnostics | Cat#: 459911 | |
| Commercial assay or kit | TRPA1 Probe | Advanced Cell Diagnostics | Cat#: 400211 | |
| Commercial assay or kit | PgP9.5 Probe | Advanced Cell Diagnostics | Cat#: 561861-C2 | |
| Chemical compound, drug | isosakuranetin | Extrasynthese | Cat#: 1374 | 20 µM |
| Chemical compound, drug | CIM0216 | Sigma-Aldrich | Cat#: 534359 | 1 µM |
| Chemical compound, drug | Pregnenolone sulfate | Sigma-Aldrich | Cat#: P162 | 100 µM |
| Chemical compound, drug | Mustard oil | Sigma-Aldrich | Cat#: W203408 | 100 µM |
| Chemical compound, drug | capsaicin | Sigma-Aldrich | Cat#: M2028 | 1 µM |
| Chemical compound, drug | DAPI | Thermo Fisher Scientific | Cat#: P36931 | |
| Software, algorithm | Turboreg algorithm | https://imagej.net/TurboReg | RRID:SCR_003070 | |
| Software, algorithm | CNMF-E algorithm | https://github.com/zhoupc/CNMF_E | RRID:SCR_001622 | |
| Software, algorithm | NIS software | Nikon Instruments | RRID:SCR_014329 | |
| Software, algorithm | OriginPro 2019b | Originlabs | RRID:SCR_014212 | |
| Software, algorithm | Igor Pro 8 | Wavemetrics | ||
| Other | CFA (complete freund’s adjuvant) | Sigma-Aldrich | Cat#: F5581 | (1 mg/ml) 10 µl per injection |
| Other | Glass-bottom microwell dish | MatTek | Cat#: P35G-1.5–14 C | |
| Other | Glass-bottom chamber | Fluorodish, WPI | Cat#: FD35-100 | |
| Other | DAPI | Thermo Fisher Scientific | Cat#: P36931 |