Fig. 8.

PD98059-sensitive pathway mediates AVP-induced NF-κB phosphorylation and IL-6 expression in mouse hearts. Pretreatment with PD98059 or the deletion of β-arrestin 2 abolished AVP-induced NF-κB phosphorylation (a) and IL-6 expression (b). After pretreatment with 3 mg/kg PD98059 for 1 h, mice were administered 0.5 U/kg AVP via the tail vein. The hearts were harvested to measure mRNA and NF-κB phosphorylation at 6 h after the administration of AVP. The upper panels are representative blots, and the lower panels show the data expressed as the means ± SEM of five separate experiments. *P < 0.05 vs. vehicle, ^##P < 0.01 vs. AVP alone