Fig. 8.

Depletion of Nrf2 partly abrogated the inhibition of ECM components and increase of antioxidant enzymes by AB38b. a Western blot analysis of collagen IV and laminin in the different groups of mesangial cells. b–c The densitometric analysis of the Western blots. The collagen IV b and laminin c signals were normalized to the β-actin signals for the same samples to determine the fold-change relative to the control, the expression level of which was set as 1. d–e CAT activity d, GSH levels e, and SOD activity f in the different groups of mesangial cells. The data are expressed as the mean ± SD, n = 3. *P < 0.05, **P < 0.01 vs. HG + si-Veh; ^#P < 0.05, ^##P < 0.01 vs. HG + si-Nrf2