Fig. 8.

Inhibition of AMPK by compound C partly reversed the protective effects of NADPH against myocardial I/R. Neonatal rat cardiomyocytes were treated as described in Figs. 1 and 4. N(L) = 60 nM, N(H) = 500 nM. Compound C (CpC, 5 μM) was added to the medium at the onset of reperfusion. a CpC reduced AMPK phosphorylation in cardiomyocytes. b CpC abolished NADPH-induced protective effects in cardiomyocytes. The bar represents the mean ± SD, n = 3 independent cultures. ^*P < 0.05, ^***P < 0.001 compared with OGD/R, ^###P < 0.001 compared with N(L), ^&&&P < 0.05 compared with N(H)