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. 2020 Apr 19;66(4):299–306. doi: 10.1262/jrd.2020-033

Table 1. Laboratory species yielding offspring following the transfer of cryopreserved embryos [4].

Species Cryopreservation method Cryoprotective agents Authors Year References
Mouse Slow freezing 1 M DMSO Whittingham et al. 1972 [5]
Rabbit Slow freezing 1.6 M DMSO Bank et al. 1974 [6]
Rat Slow freezing 1.5 M DMSO Whittingham et al. 1975 [7]
Mastomys Vitrification (2-step) 20% EG+F+S and 40% EG+F+S Mochida et al. 1998 [14, 15]
Hamster Vitrification (2-step) 10% DMSO+10% EG and 20% DMSO+20% EG+F+S Lane et al. 1999 [16, 17]
Mongolian gerbil Vitrification (2-step) 20% EG+F+S and 40% EG+F+S Mochida et al. 1999 [18, 19]

DMSO, dimethyl sulfoxide; EG, ethylene glycol; F, Ficoll; S, sucrose.