Figure 6.

Inhibition of neuronal CMKLR1 attenuates tau hyperphosphorylation induced by high glucose treatment in SH-SY5Y cells. A, Representative immunofluorescence images of CMKLR1 (green) in SH-SY5Y cells. Scale bar, 50 μm. B, Representative Western blots showing tau hyperphosphorylation at Ser199, Thr205, and Ser396 from SH-SY5Y treated with 5, 15, or 30 mm glucose for 12 or 24 h. Quantification of the immunoreactivity of the blots, normalized against total tau (C) and β-actin (D). E, Representative Western blots showing tau hyperphosphorylation at Ser199 and Ser396 from SH-SY5Y incubated with 5 or 30 mm glucose for 24 h, with or without a 1 h pretreatment with C15 (1 or 10 µm). Quantification of the immunoreactivity of the blots, normalized against total tau (F) and β-actin (G). Data are mean ± SEM from three separate experiments, each in duplicate or triplicate. *p < 0.05; **p < 0.01; ***p < 0.001; compared with 5 mM glucose treatment group. ^$p < 0.05; ^$$p < 0.01; compared with 30 mm glucose treatment group. Glu: glucose.