Table 1.
Primary antibodies used in this study
| Antibody | Type | Specificity | Phosphorylation sites | Reference/source |
|---|---|---|---|---|
| Tau5 | Monoclonal | Tau (total) | Affinity Bioreagents | |
| pT199 | Polyclonal | P-tau | Ser199 | Invitrogen |
| pT205 | Polyclonal | P-tau | Thr205 | Invitrogen |
| pS396 | Polyclonal | P-tau | Ser396 | Invitrogen |
| pS404 | Polyclonal | P-tau | Ser404 | Invitrogen |
| Tau1 | Monoclonal | Tau (non-phosphorylated) | Merck | |
| Tau-13 | Monoclonal | Human tau | Tau15-25 | Biolegend |
| GFAP-Cy3TM | Monoclonal | GFAP | Sigma Millipore | |
| anti-β-actin | Polyclonal | β-actin | Sigma Millipore | |
| Iba1 | Polyclonal | Iba1 | FUJIFILM Wako Pure Chemical | |
| GFAP | Polyclonal | GFAP | Cell Signaling Technology | |
| CMKLR1/AF488 | Polyclonal | CMKLR1 | Bioss | |
| MAP2 | Polyclonal | MAP2 | Sigma Millipore | |
| APP | Polyclonal | APP | Cell Signaling Technology | |
| Aβ | Polyclonal | Aβ | Cell Signaling Technology |