Figure 3.

DREADD-mediated inhibition of ATN cortex impairs spatial working memory. A, Sagittal schematic of rat forebrain. B, AAV5-hM4D(Gi)-mCherry expression in ATN (coronal section). All animals displayed robust expression within the ATN and their efferent targets (see also Fig. 4). C, DREADD activation did not reliably disrupt spatial working memory on the standard version of the alternation task, but performance was impaired on the modified version (maze rotation). The DREADD group was selectively impaired relative to their performance under saline (p < 0.001) as well as to the performance of controls under clozapine (p < 0.005). Plots represent mean percentage correct. Error bars indicate SEM. *denotes statistically significant differences.